علوم زیستی دریا
Negin Derakhshesh; Abdolali Movahedinia; Negin Salamat; Mahmoud Hashemitabar; Vahid Bayati
Abstract
The liver is one of the most important organs of animals that know as a detoxifying and filtering toxin in the body and the most studies was used the target organ. In peresent study, hepatocytes were isolated from 8 immature grouper fish (equal to the number of male and female). Then, anesthetized fish ...
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The liver is one of the most important organs of animals that know as a detoxifying and filtering toxin in the body and the most studies was used the target organ. In peresent study, hepatocytes were isolated from 8 immature grouper fish (equal to the number of male and female). Then, anesthetized fish and the head was separated from the body. The fish wiped with 70% etanol alchol. The liver was removed was cuted with scissors. Finally, the cells digested by collagen type 4. Then, the cells incubated in 30^C and 20% FBS+ITS during two weeks. The doses of pollutant (100 µM, 2*100 µM, 3*100 µM) added to samples, then were studied in 0, 6, 12 and 24 time. The morphological analysis exhibited that the cell death incubated by B[a]P polluted by concentration of 100 µm results in 50% of apoptosis cell death. Our finding from this study showed that using lower dosage of B[a]P during 24h treatment period cause inhibition of proliferation in time and dose dependent manner and higher doasage cause lysis and necrosis.
علوم زیستی دریا
alireza safahieh; yaqub jaddi; abdol ali movahedinia; ali hallajian; sohrab dajandian
Abstract
The actue toxicity test was performed instatic renewal system based on the standared method Proposed by OECD during 96 h, in which physicochemical parameters of test water including pH, dissolved oxygen and Temprature were daily monitored. to determine the lethal range of diazinon of fish a range finding ...
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The actue toxicity test was performed instatic renewal system based on the standared method Proposed by OECD during 96 h, in which physicochemical parameters of test water including pH, dissolved oxygen and Temprature were daily monitored. to determine the lethal range of diazinon of fish a range finding test was carried out in aquariums coutaing 10 L of test solution. The acute toxicity test was then preformed in 5 treatment in triplicats. The obtained data were analyzed using probit analysis with %5 confidence limit. acording to the results, 24 h LC50, 48 h LC50, 72 h LC50 and 96 h LC50 of diazinon on common seabream was 10/974, 10/391, 9/134 and 7/316 respectively. The Maxium Acceptable Toxicant Concentration (MATC) of diazinon on common seabream was 0/073 mg/l and the Lowest observed effect concentration wich is equal to 96 h LC50 was 2.63 mg/l. The results indicated that compared to other fishes is more resistant against diazinon . in addition diazinon lethal toxicity takes place in a waide range of toxicant concentration.
علوم زیستی دریا
Mohsen Mohammadi Galangash; Abdolali Movahedinia; Zahra Bozorgpanah Kharat
Abstract
The microbial pollutions and nutrient matters of the southern part of Caspian Sea is to be under close scrutiny as it is vital to tourist health and environmental conservation.In this research, some physico-chemical factors, nutrient matters, oxygen demand parameters and microbial indices were investigated. ...
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The microbial pollutions and nutrient matters of the southern part of Caspian Sea is to be under close scrutiny as it is vital to tourist health and environmental conservation.In this research, some physico-chemical factors, nutrient matters, oxygen demand parameters and microbial indices were investigated. Sampling from nine sites along Astara to Chaboksar coastline were conducted with five replicates. The mean of temperature, Salinity, pH, phosphate, nitrate, FC, TC, BOD5 and COD were respectively obtained as 22.48°C, 8.5ppt, 8.27, 0.43mg/l, 2.21mg/l, 144MPN/100ml, 626.9MPN/100ml, 3.58mg/l and 9.43 mg/l. Maximum levels of nutrient matters were measured at Anzali and Kiyashahr sites. Results showed there are positive correlations between nutrient matters as well as COD with microbial indices. The average of microbial indices at nine sampling sites were higher than the permissible level of national and international organizations for swimming places. Due to high level of microbial pollution at Anzali and Chobar sampling sites, the swimming places of these areas require specific administration.
Rahman Alimi; Ahmad savari; Abdolali Movahedinia; Mohammad Zakeri; Negin salamat
Abstract
The Brown banded Bamboo shark is a benthic shark and unlike most of shark, to attract the oxygen in the water no need to move constantly. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) were caught from Dervish's Creek in the northern Persian Gulf. Certain ...
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The Brown banded Bamboo shark is a benthic shark and unlike most of shark, to attract the oxygen in the water no need to move constantly. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) were caught from Dervish's Creek in the northern Persian Gulf. Certain sections of the gill tissue removed and immediately fixed in Bouin’s solution. Then embedded the samples in paraffin and sectioned into 5 micrometer microscopic and stained with hematoxylin and eosin. In order to evaluate the comprehensive, sagittal and cross-section of gill tissue were studied. Microscopic results of cross-section indicated that the gill tissue structures including, filaments and lamella sections revealed. In addition, a variety of cells such as columnar cells and chloride cells (MR) were also detected. According to the results obtained for the cross section, The cell aggregates, named follicularlyarranged NKA-rich cells (follicular NRCs), were found in the gills septum. Therefore, the Brown banded Bamboo shark has three types of NKA-rich cells in the gill: (1) NKA-rich MR cells on the filament and lamella, (2) NKA-rich solitary cells (solitary NRCs) and (3) follicular NRCs on the gill septum.
علوم زیستی دریا
mohsen heidari; hossein zolgharnine; n s; a m
Abstract
The purpose of this study was to evaluate the antioxidant properties of hydroalchoholic extractions of three species of green, brown and red algae. The highest and the lowest total phenol contents was in L. snyderia (113/9±0/69 mg gallic acid per gram of extract) and the green algae E. intestinalis ...
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The purpose of this study was to evaluate the antioxidant properties of hydroalchoholic extractions of three species of green, brown and red algae. The highest and the lowest total phenol contents was in L. snyderia (113/9±0/69 mg gallic acid per gram of extract) and the green algae E. intestinalis (72/36±6/05 mg gallic acid per gram of extract) respectively. The highest values of total flavonoid was founded in the red algae L. snyderia (41/05±1/95 mg Rutin per gram of extract) however, the lowest values was (12/7±0/41 mg Rutin per gram of extract) in the brown alga C. trinodis. L. snyderia showed the most antioxidant activity, and C. trinodis had lowest amounts of antioxidant potential by the radical, azinobis ethylen benz thiazoline sulphonic acid (ABTS) test. there were significant differences between anti-oxidant activities of algae according the ABTS test.
علوم زیستی دریا
mohsen heidari; hossein zolgharnine; nasrin sakhaei; abdolali movahedinia; a m
Abstract
The purpose of this study was to evaluate of bio diversity and density of brown algae in coast of Bushehr province in the Persian Gulf during warm and cold seasons. The most and lowest density of brown algae was in the spring (33±20Number in m2) and in autumn(17± 9Number in m2) respectively. The highest ...
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The purpose of this study was to evaluate of bio diversity and density of brown algae in coast of Bushehr province in the Persian Gulf during warm and cold seasons. The most and lowest density of brown algae was in the spring (33±20Number in m2) and in autumn(17± 9Number in m2) respectively. The highest density was belonged to species of Cystoserria myrica with(41±25)and it was dominant species among the six species of brown algae were sampled. The most of Margalef index(0/438) was observe at the mid, and mid litoral zone. The maximum value of the Shannon index(0/94) was belonged to the low, mid litoral zone. Most of species richness and dominance was revealed at autumn and highest indices of Shannon(0/76) was at spring. Brown algae in the spring had the most density (33±20/86 Number in m2). Species richness was detected the highest and lowest in Nuclear power Plant and Ganaveh stations in two seasons respectively. Higher levels of dominance and low diversity was in within the tidal zone.
علوم زیستی دریا
Rahman Alimi; Ahmad Savari; Abdolali Movahedinia; Mohammad Zakeri; Negin Salamat
Abstract
Cartilaginous fishes were used urea for osmoregulation of their internal fluids. There are several studies on osmoregulation of sharks but investigate the role of reproduction season and body lenght on the concentration of osmolytes is necessary. In this investigation, 36 individual of Brown banded Bamboo ...
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Cartilaginous fishes were used urea for osmoregulation of their internal fluids. There are several studies on osmoregulation of sharks but investigate the role of reproduction season and body lenght on the concentration of osmolytes is necessary. In this investigation, 36 individual of Brown banded Bamboo sharks (Chiloscyllium punctatum) in both autumn (before reproduction) and spring (period of reproduction) seasons were caught from Moussa Creek located at the northern of Persian Gulf. After weighing, biometry, sex determination and the numbering of sharks. Electrolyte analyzer and auto analyzer were used to measuring electrolyte and urea concentration. The results showed that blood osmolytes of sharks in the male and female sex were no significant differences (P>0/05). But, there was a significant difference between the concentrations of these substances in the autumn and spring season (P
Abstract
Among more than 600 fish species in Persian Gulf, Soles are considered as one of the most economically important species. These fish is different from other fishes in morphological , functional and structural organs and like many other flatfish are less well known. The present study was designed to investigate ...
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Among more than 600 fish species in Persian Gulf, Soles are considered as one of the most economically important species. These fish is different from other fishes in morphological , functional and structural organs and like many other flatfish are less well known. The present study was designed to investigate the histological structure of gonads of Sole (Euryglossa orientalis) in two breeding and rest seasons. 40 Sole fish were collected from Zangi estuary (located in Mussa creek in the north of Persian Gulf) in early July and early October(non- breeding and breeding seasons) 2013. After biometry, gonadal tissues were dissected and weighed to determine Gonadosomatic Index (GSI). Then fixed in Bouin 's solution. Histological specimens were transported to the histological laboratory. Tissue sections were prepared according to conventional methods include dehydration, clearing and paraffin. And then were cut down to 5 micron sections and stained. These tissue sections were studied by light microscopy . The result showed that different stages of germ cells in both male and female gonads. Although in breeding season mature cells were noticed, GSI index of testis and ovary in Sole showed significant difference between the two seasons.
bita archangi
Abstract
In this study, effects of BPA on erythrocytic nuclear abnormalities (ENA) stimulus (MN Test) and liver DNA integrity (DNA Unwinding Assay), in male yellowfin seabream (Acanthopagrus latus) were investigated. For this reason, fish received intraperitoneal injections during a period of 2 weeks with 10, ...
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In this study, effects of BPA on erythrocytic nuclear abnormalities (ENA) stimulus (MN Test) and liver DNA integrity (DNA Unwinding Assay), in male yellowfin seabream (Acanthopagrus latus) were investigated. For this reason, fish received intraperitoneal injections during a period of 2 weeks with 10, 50, 100 and 150 µg g-1 week-1 of BPA dissolved in coconut oil. Solvent controls received the coconut oil whereas controls were not injected. The fish were sampled on day 0, 7 and 14. In order to evaluate cytotoxicity of BPA presented in blood, the erythrocytic nuclear abnormalities (ENA) frequency in yellowfin seabrem was determined. Our results demonstrated a significant increase in fish micronuclei frequency after the treatment with bisphenol A in comparison to the control groups and in dose dependent manner. In addition, the rate of liver DNA integrity was tested using the DNA alkaline unwinding assay. Results showed a decrease in the rate of liver DNA integrity in treated fishes after 7 and 14 days of BPA exposure in comparison to the control group. In conclusion, results of the current project indicated that BPA has high genotoxic and/or cytogenotoxic potential. It could be concluded that Micronucleus test and DNA strand breaks can be used as sensitive cellular and molecular indicators of exposure to genotoxic BPA.
Sara Rastgar; Abdolali Movahedinia
Volume 11, Issue 4 , March 2013, , Pages 25-31
Abstract
The present study carried out to assess the effects of Benzo-α-Pyren (BaP) as a serious member of Polycyclic Aromatic Hydrocarbons (PAH) on the plasma levels of cortisol and triiodothyronine (T3) hormones in male Seabream, Acanthopagrus latus (Sparidea). To assess the acute effects of BaP on the ...
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The present study carried out to assess the effects of Benzo-α-Pyren (BaP) as a serious member of Polycyclic Aromatic Hydrocarbons (PAH) on the plasma levels of cortisol and triiodothyronine (T3) hormones in male Seabream, Acanthopagrus latus (Sparidea). To assess the acute effects of BaP on the endocrine system, a group of fish was injected with dissolved BaP in sunflower oil (50mg/kg BaP in 2µg/l oil according to the body weight). Other fishes were injected with 2µg/l body weight as controls. Blood samples from both groups collected 3 hours after injection. In another protocol, the chronic effects of BaP on the plasma levels of cortisol and T3 were assessed. For this reason a group of fish was implanted with 50mg/kg BaP in 10µl/g coconut oil in respect to each fish body weight. As the previous protocol a control group was implanted with the same amount of oil without the BaP. Blood samples collected from both implanted groups (treatment and control) after 72 hours. According to the results, plasma levels of cortisol in yellowfin Seabream increased during both short time and long time exposure but T3 levels in the plasma decreased during the chronic stress of BaP exposure. It seems the cortisol levels are elevating to provide more energy requirement under the acute and chronic stress conditions by direct effect on glyconeogenesis and fast blood sugar level to be consumed by cells. T3 levels may be affected by inhibitory effect of Aryl hydrocarbon receptors (AhRs) which decreased it during long term exposure.
Abdolali Movahedinia; Zahra Yarahmadi; Sara Rastgar
Volume 11, Issue 3 , November 2012, , Pages 52-62
asieh mirali; abdolali movahhedinia; rahim abdi; amirparviz salati
Volume 10, Issue 4 , September 2012, , Pages 15-21
Abstract
Water and electrolyte balance is vital for aquatic organisms in both hyper and hypo osmotic environments. Kidneys as excretory organs play an important role in osmoregulation and body fluid homeostasis. In this study a total number of 180 Sobayti (Teleost: Sparidentex hasta) were exposed to different ...
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Water and electrolyte balance is vital for aquatic organisms in both hyper and hypo osmotic environments. Kidneys as excretory organs play an important role in osmoregulation and body fluid homeostasis. In this study a total number of 180 Sobayti (Teleost: Sparidentex hasta) were exposed to different salinities to assess changes in the kidney structure and alterations in plasma levels of electrolytes, glucose and cortisol. Salinity challenges were examined for a period of 14 days in four experimental groups: 40ppt as control and 5, 20 and 60 ppt as other experimental treatments. 24 hours after salinity challenge, significant changes were observed in the lumen diameter of the primary proximal tubule at the sections from trunk kidney in both 5 and 20 ppt conditions (P<0.05). Lumen diameter of the second part of the proximal tubule did not differ in any of the experimental salinities (P>0.05).However, lumen diameter of the distal tubule of nephron in the trunk kidney, increased significantly in 60 ppt treatment 48 hours from starting the experiment (P<0.05). The thickness of the epithelium tubule in both trunk and tail parts showed no significant differences during the adaptations (P>0.05). According to the results, histomorphological changes in the different parts of the kidney returned to the basic structure with in 24 to 48hours after exposure to different salinities.
Sara Sara Rastegar1; Abdolali Movahedinia; Zahra Yarahmadi
Volume 10, Issue 3 , September 2012, , Pages 40-47
Abstract
The present study carried out to assess the effects of Benzo-α-Pyrene (BaP) as a serious member of Polycyclic Aromatic Hydrocarbons (PAH) on the plasma levels of cortisol and triiodothyronine (T3) hormones in male Seabream, Acanthopagrus latus (Sparidea). To assess the acute effects of BaP on the ...
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The present study carried out to assess the effects of Benzo-α-Pyrene (BaP) as a serious member of Polycyclic Aromatic Hydrocarbons (PAH) on the plasma levels of cortisol and triiodothyronine (T3) hormones in male Seabream, Acanthopagrus latus (Sparidea). To assess the acute effects of BaP on the endocrine system, a group of fish was injected with dissolved BaP in sunflower oil (50mg/kg BaP in 2µg/l oil according to the body weight). Other fishes were injected with 2µg/l body weight as controls. Blood samples from both groups collected 3 hours after injection. In another protocol, the chronic effects of BaP on the plasma levels of cortisol and T3 were assessed. For this reason a group of fish was implanted with 50mg/kg BaP in 10µl/g coconut oil in respect to each fish body weight. As the previous protocol a control group was implanted with the same amount of oil without the BaP. Blood samples collected from both implanted groups (treatment and control) after 72 hours. According to the results, plasma levels of cortisol in yellowfin Seabream increased during both short time and long time exposure but T3 levels in the plasma decreased during the chronic stress of BaP exposure. It seems the cortisol levels are elevating to provide more energy requirement under the acute and chronic stress conditions by direct effect on glyconeogenesis and fast blood sugar level to be consumed by cells. T3 levels may be affected by inhibitory effect of Aryl hydrocarbon receptors (AhRs) which decreased it during long term exposure.